Duties

  • Mammalian cell culture and banking, cell transfection of DNA plasmids / siRNAs / ASOs, and lentiviral cell transduction
  • RNA/DNA/protein extraction from mammalian cell lines for immunoblotting, qRT-PCR and/or others
  • Design and execute siRNA, RT-qPCR and reporter assays for gene expression and splice variant detection
  • Cell-based assays for small molecule testing in miniaturized format: proliferation, toxicity, imaging and/or others
  • Help design CRISPR genome editing experiments
  • Ensure that safety guidelines are strictly followed at all times and keep an orderly work environment
  • Work closely with lab head and biology/chemistry group leaders to design, develop, execute and analyze experiment protocols
  • Participate in the onboarding and training of new team members for defined techniques and instruments
  • Record all data and results in electronic format with accuracy and responsibility
  • Summarize obtained data in an organized and presentable fashion, develop PowerPoint presentations for regular laboratory meetings and external presentations
  • Manage various databases

 Requirement

  • Bachelor/equivalent technical education or Master of Science
  • Excellent understanding of modern molecular biology (RT-qPCR, siRNA) and cellular assay tools
  • Experience working with miniaturized systems (384-well format)
  • Proficiency in MS Office package and GraphPad prism
  • Good command of the research literature and databases
  • Excellent planning and organizing skills, able to manage multiple tasks simultaneously and set priorities
  • Flexible, efficient, able to work independently and proactively with a problem-solving attitude and within in a fast-paced team environment
  • Fluent in English (spoken and written), any further languages are an asset